Single-cell expression profiling reveals dynamic flux of cardiac stromal, vascular and immune cells in health and injury

Nona Farbehi, Ralph Patrick, Aude Dorison, Munira Xaymardan, Vaibhao Janbandhu, Katharina Wystub-Lis, Joshua WK Ho, Robert E Nordon Is a corresponding author, Richard P Harvey.
Last updated on Jul 18, 2021
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Pinto Lab - Baker Heart and Diabetes Institute

Abstract

Besides cardiomyocytes (CM), the heart contains numerous interstitial cell types which play key roles in heart repair, regeneration and disease, including fibroblast, vascular and immune cells. However, a comprehensive understanding of this interactive cell community is lacking.

Here, authors have performed single-cell RNA-sequencing on the total non-CM fraction and enriched (Pdgfra-GFP+) fibroblast lineage cells from murine hearts at days 3 and 7 post-sham or myocardial infarction (MI) surgery. This data highlight non-linear dynamics in myeloid and fibroblast lineages after cardiac injury, and provide an entry point for deeper analysis of cardiac homeostasis, inflammation, fibrosis, repair and regeneration.

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Name Sample Attributes Variables Assay
ENA Source Name organism strain age developmental stage sex organism part genotype fraction time fraction injury Assay Name
ERR2931607 MI_day3_TIP_S2 Mus musculus C57BL/6J 8 (week) adult male cardiac ventricle B6.129S4-Pdgfratm11(EGFP)Sor/J total interstitial non-myocyte cell 3 (day) total interstitial non-myocyte cell myocardial infarction MI_day3_TIP_S2_L001
ERR2931608 MI_day3_TIP_S2 Mus musculus C57BL/6J 8 (week) adult male cardiac ventricle B6.129S4-Pdgfratm11(EGFP)Sor/J total interstitial non-myocyte cell 3 (day) total interstitial non-myocyte cell myocardial infarction MI_day3_TIP_S2_L002
ERR2931609 MI_day3_TIP_S2 Mus musculus C57BL/6J 8 (week) adult male cardiac ventricle B6.129S4-Pdgfratm11(EGFP)Sor/J total interstitial non-myocyte cell 3 (day) total interstitial non-myocyte cell myocardial infarction MI_day3_TIP_S2_L003
ERR2931610 MI_day3_TIP_S2 Mus musculus C57BL/6J 8 (week) adult male cardiac ventricle B6.129S4-Pdgfratm11(EGFP)Sor/J total interstitial non-myocyte cell 3 (day) total interstitial non-myocyte cell myocardial infarction MI_day3_TIP_S2_L004
ERR2931611 MI_day7_TIP_S2 Mus musculus C57BL/6J 8 (week) adult male cardiac ventricle B6.129S4-Pdgfratm11(EGFP)Sor/J total interstitial non-myocyte cell 7 (day) total interstitial non-myocyte cell myocardial infarction MI_day7_TIP_S2_L001
ERR2931612 MI_day7_TIP_S2 Mus musculus C57BL/6J 8 (week) adult male cardiac ventricle B6.129S4-Pdgfratm11(EGFP)Sor/J total interstitial non-myocyte cell 7 (day) total interstitial non-myocyte cell myocardial infarction MI_day7_TIP_S2_L002
ERR2931613 MI_day7_TIP_S2 Mus musculus C57BL/6J 8 (week) adult male cardiac ventricle B6.129S4-Pdgfratm11(EGFP)Sor/J total interstitial non-myocyte cell 7 (day) total interstitial non-myocyte cell myocardial infarction MI_day7_TIP_S2_L003
ERR2931614 MI_day7_TIP_S2 Mus musculus C57BL/6J 8 (week) adult male cardiac ventricle B6.129S4-Pdgfratm11(EGFP)Sor/J total interstitial non-myocyte cell 7 (day) total interstitial non-myocyte cell myocardial infarction MI_day7_TIP_S2_L004
ERR2931615 Sham_day7_TIP_S2 Mus musculus C57BL/6J 8 (week) adult male cardiac ventricle B6.129S4-Pdgfratm11(EGFP)Sor/J total interstitial non-myocyte cell 7 (day) total interstitial non-myocyte cell sham Sham_day7_TIP_S2_L001
ERR2931616 Sham_day7_TIP_S2 Mus musculus C57BL/6J 8 (week) adult male cardiac ventricle B6.129S4-Pdgfratm11(EGFP)Sor/J total interstitial non-myocyte cell 7 (day) total interstitial non-myocyte cell sham Sham_day7_TIP_S2_L002
ERR2931617 Sham_day7_TIP_S2 Mus musculus C57BL/6J 8 (week) adult male cardiac ventricle B6.129S4-Pdgfratm11(EGFP)Sor/J total interstitial non-myocyte cell 7 (day) total interstitial non-myocyte cell sham Sham_day7_TIP_S2_L003
ERR2931618 Sham_day7_TIP_S2 Mus musculus C57BL/6J 8 (week) adult male cardiac ventricle B6.129S4-Pdgfratm11(EGFP)Sor/J total interstitial non-myocyte cell 7 (day) total interstitial non-myocyte cell sham Sham_day7_TIP_S2_L004
mouse heart